- Cells were harvested and protein extracts were prepared for immunoprecipitation and immunoblotting with the indicated antibodies.
- The samples were processed by immunoprecipitation followed by immunoblotting.
- Fraction purities were assessed by a combination of Coomassie staining and immunoblotting of electrophoretically separated proteins.
- The specificity of this antibody towards wood peroxidases was confirmed by immunoblotting.
- Extraction of total soluble protein from tobacco leaves and immunoblotting using the above antibody was carried out according to standard protocols.