- In the normal lymphoid ontogeny, genomic recombinations occur in a tightly regulated sequence and require an enzyme called recombinase.
- They probably integrate via double-stranded, closed-circle DNA intermediates through the action of an encoded recombinase related to the site-specific integrase.
- Originally FIS was discovered as a protein that enhances the inversion reactions of several recombinases.
- Therefore, continuous supply of recombinase would promote the generation of homozygous cells.
- All of these studies suggest that recombinase proteins, in their current form, have undergone selective pressure to limit their activities.
- In this process, the oligonucleotide is believed to hybridize to the target site through the action of a DNA recombinase and, once bound, DNA repair enzymes act to excise the nucleotide, replace it, and revert the gene to wild-type status.