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- Test solutions were removed from the perfusion chamber by suction via a vacuum pump.
- Many scientists, particularly Hobbes, had argued that a vacuum could not exist and claimed that Boyle's results obtained with the vacuum pump must be the result of some as yet undiscovered force.
- Negative pressure is generated by a vacuum pump.
- The filter comprises a slowly rotating drum, semi-immersed in the product to be filtered, covered in a fine stainless steel mesh, and fitted with an internal vacuum pump.
- It was transferred into a sealed flask and air was pulled out by a vacuum pump for a few minutes until bubbles stopped forming.